26 August 2008
10:01:00 PM
Alright, answers to all of your queries (:
YvonneThe 20ul is the initial amt used for the test to see which one has positive result. Then the 5ul or 10ul is not dilution. It is just to see whether the reaction is still positive at a lower volume, this means that the patient has a high amount of antibodies against the antigen.
For your lab, why do you need the waterbath? And how this works? Isit the Rickettsia IgG or IgM detection?
BenjaminYour question is abit difficult, but I've found answer to your question(: Basically, the O and H letters is a classification for Salmonella species, and the
O antigens stands for the polyssacharides and the lipopolyssacharides found on the
outer membrane of the bacteria cell.
H antigen stands for the proteins related to the
flagellum of the bacteria. And in this H antigen, there are still groupings like Group A, B, C etc, to type the different stage of Salmonella species, whether is it in motile or non-motile stage (since it is the antigen for flagellum of the bacteria)
Xin YiYes you are correct, an example would be Proteus OX19 which has the same o-polysaccharides as the highlt pathogenic bacteria
Rickettsia prowazekii. It is 1:80 because 1:20 and 1:40 is the same as they show only positive cases in the 20ul sample (lowest titre). 1:80 then it is significant enough to be recorded in the 10ul sample. (:
Hope all you all understand and it helps (:
Thank you for all of your support!
LESLIE
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