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Gladys Chia
Chew Yu Mei
Yvonne Teo
Cornelyus Ng
Lau Leslie

19 October 2008

2:49:00 PM

Hello everyone!

This would be my last post for the whole sip experiment. I am going to share with you guys about this analyte, PROCALCITONIN. My MP is about the evaluation of procalcitonin on Cobas e6000 together with zhaorong and yuxuan. Since yuxuan had blogged about our evaluation parameters, I’ve decided to blog about the current method/instrument my lab is using to analyze procalcitonin.

BRAHMS KRYPTOR!

http://www.surrey.ac.uk/MHRA/Pages/GuildfordEvaluations/PreviousReports/Reports/Brahms%20Kryptor.html%

The Procalcitonin assay on the Kryptor analyzer, is for the quantitative analysis of Procalcitonin (PCT) in human serum as a potential biomarker of severe bacterial infections.

Procalcitonin is a 116 amino acid precursor of calcitonin. Although it I a prohormone under normal conditions, it is induced several fold in systemic bacterial infection and sepsis to elevated levels found in blood. However, in non-specific inflammatory disease and viral infections, levels of PCT remain relatively low. The PCT molecule has a half-life of about 24 hours, making it a very stable protein.

Typically, increased levels of PCT are observed 2 to 3 hours with septic infection and as the infection resolves, PCT levels will return to the normal range of below 0.5ug/L.

Principle of Kryptor
The homogenous immunoassay employs TRACE (time-resolved cryptate emission) technology that is based on a non-radiative transfer of energy. The transfer takes place between two fluorescent tracer (a donor europium cryptate and an acceptor XL565) conjugated antibodies. With the formation of an immuno-complex, excitation allows energy transfer between the close proximity tracers with amplified signal at 665nm.

http://www.procalcitonin.com/default.aspx?tree=_4_0_0&key=kryptor2

Patient preparation: No special preparation needed.

Type: Serum preferred. However, with neonatal samples EDTA-plasma
is allowed. Avoid haemolysis, icterus and lipaemic condition of sample.
Do not use citrate-plasma.

Handling conditions: Serum should be stored in the refrigerator if analysis cannot be
performed on the day of receipt. Samples are stable at 2-8 degree
Celsius for up to 7 days. Frozen samples should not be subjected to
more than one freeze-thaw cycle.

Controls: 2 levels of controls are run every morning before specimens are
analysed.

Calibrations: Once every 2 weeks.

Procedures:
1. Label the request form and the test tube with the correct barcode.
2. Spin down the plain tubes if required.
3. Enter the barcode number, patient’s name and ic number in the “worklist” of the analyzer.
4. Select the test “Procalcitonin”.
5. Load the specimens into the analyzer.
6. Press [start]. (results will be out in 19 minutes)

Chew Yu Mei
TG01

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